Protocols & Software

Lab Protocols

• Protein extracts in TCA
• FACS analysis of yeast samples
• Yeast mating with diploid isolation
• Yeast nuclear staining
• One-Step gene disruption/tagging
• Fixation of cells with GFP-tagged proteins
• High efficiency yeast transformation

Novel yeast reference genome (SacCerRep)

You can download the yeast reference genome used in all my works here. As described, this improves the mapping metrics and allows access to the repetitive genome.

Software

• augur-fermentorum: a collection of tools to automatically analyse yeast whole-genome sequencing data used in the Nature paper (Puddu, 2019)
• Docker images used for the analysis pipeline

Ad-hoc scripts

• Protein Mutation Maps: a tool used to map mutations on the protein primary structure used in the Scientific Reports paper (Herzog, 2018)